Cytotoxic activities of Helichrysum arenarium on ECV 304 and Ishikawa cells
DOI:
https://doi.org/10.62482/pmj.12Keywords:
Helichrysum arenarium, cytotoxicity assay, MDA assay, GC-MS, Ishikawa cellsAbstract
Introduction: In this study, the cytotoxic activity of Helichrysum arenarium plant on various human cells were examined.
Methods: Cytotoxicity results were found to be the most significant Helichrysum arenarium plant with DMSO extract ECV 304 (human endothelial cell line) and Ishikawa cells (human endometrial adenocarcinoma cells) and lipid peroxidation (MDA) assay was performed. The most significant extract dose was determined according to the experimental results. Also, the essential oils of immortelle extracts were analyzed using gas chromatography-mass spectrometry.
Results: Cell viability and MDA levels were decreased by the extract of Helichrysum arenarium both in Ishikawa cells and ECV304 cells at the concentration level of 500 µg/mL. 2-Palmitoylglycerol and palmitic acids were found as the major essential oils in DMSO extracts of Helichrysum arenarium.
Conclusion: Helichrysum arenarium may show an antioxidant activity in vitro.
References
Sawilska AK. Population structure of sandy everlasting Helichrysum arenarium (L.) moench in relation to the advancement of the secondary succession of swards on sandy soils. Infrastructure and Environment. 2019;169-74. https://doi.org/10.1007/978-3-030-16542-0_23
World Health Organization (WHO). WHO monographs on medicinal plants commonly used in the Newly Independent States (NIS). Libros Digitales-World Health Organization (WHO). 2010.
Eshbakova KA, Aisa HA. Components of Helichrysum arenarium. Chemistry of Natural Compounds 2009; 45:929–30. https://doi.org/10.1007/s10600-010-9462-3
Czinner E, Lemberkovics É, Bihátsi-Karsai E, Vitányi G, Lelik L. Composition of the Essential Oil from the Inflorescence of Helichrysum arenarium(L.) Moench. the Journal of Essential Oil Research 2000; 12:728–30. https://doi.org/10.1080/10412905.2000.9712202
Mathekga ADM, Meyer JJM, Horn MM, Drewes SE. An acylated phloroglucinol with antimicrobial properties from Helichrysum caespititium. Phytochemistry 2000;53:93-6. https://doi.org/10.1016/s0031-9422(99)00424-0
Albayrak S, Aksoy A, Sağdiç O, Budak Ü. Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey. Turk. J. Biol. 2010;34(4):463-473. https://doi.org/10.3906/biy-0901-4
Carini M, Aldini G, Furlanetto S, Stefani R, Facino RM. LC coupled to ion-trap MS for the rapid screening and detection of polyphenol antioxidants from Helichrysum stoechas J. Pharm. Biomed. Anal. 2001; 24:517-26. https://doi.org/10.1016/S0731-7085(00)00431-3
Tepe B, Sokmen M, Akpulat HA, Sokmen A. In vitro antioxidant activities of the methanol extracts of four Helichrysum species from Turkey. Food Chem. 2005; 90:685-9. https://doi.org/10.1016/j.foodchem.2004.04.030
Gradinaru AC, Silion M, Trifan A, Miron A, Aprotosoaie AC. Helichrysum arenariumsubsp.arenarium: phenolic composition and antibacterial activity against lower respiratory tract pathogens. Nat. Prod. Res. 2014; 28:2076-80. https://doi.org/10.1080/14786419.2014.924931
Musabayane CT, Kamadyaapa DR, Gondwe M, Moodley K, Ojewole J a O. Cardiovascular effects of Helichrysum ceres S Moore [Asteraceae] ethanolic leaf extract in some experimental animal paradigms. Cardiovasc. J. Afr. 2008;19(5):246
Czinner E, Hagymási K, Blázovics A, Kéry Á, Szőke É, Lemberkovics É. The in vitro effect of Helichrysi flos on microsomal lipid peroxidation. J. Ethnopharmacol. 2001;77:31-5. https://doi.org/10.1016/S0378-8741(01)00258-6
Lorenzo JLF. Plants as a source of photoprotective compounds against ultraviolet radiation-induced DNA damage. Revista Acad. Colomb. Ci. Exact. 2019;43(168):550-562. http://dx.doi.org/10.18257/raccefyn.841
Moghadam HD, Sani AM, Sangatash MM. Inhibitory effect of Helichrysum arenarium essential oil on the growth of food contaminated microorganisms. Journal of Essential Oil-Bearing Plants. 2014;17(5):911-921. https://doi.org/10.1080/0972060X.2014.890073
Kurkin VA, Zaitseva EN, Kurkina AV, Dubishchev AV, Pravdivtseva OE. Comparative Study of diuretic activity of hydroalcoholic extracts from medicinal plants containing flavonoids. Bull. Exp. Biol. Med. 2015; 159:368–371. https://doi.org/10.1007/s10517-015-2965-y
Jarzycka A, Lewińska A, Gancarz R, Wilk KA.Assessment of extracts of Helichrysum arenarium, Crataegus monogyna, Sambucus nigra in photoprotective UVA and UVB; photostability in cosmetic emulsions. J. Photochem. Photobiol. B. Biol. 2013; 128:50-7. https://doi.org/10.1016/j.jphotobiol.2013.07.029
Pırıldar S, Sütlüpınar N, Atasever B, Erdem-Kuruca S, Papouskova B, Šimánek V. Chemical constituents of the different parts of Colchicum baytopiorum and their cytotoxic activities on K562 and HL60 cell lines. Pharm Biol. 2010;48(1):32-9. https://doi.org/10.3109/13880200903029373
Cebecioglu R, Yildirim M, Akagunduz D, Korkmaz I, Tekin HO, Atasever-Arslan B, Catal T. Synergistic effects of quercetin and selenium on oxidative stress in endometrial adenocarcinoma cells. Bratislavske lekarske listy.2019;120(6):449-55. https://doi.org/10.4149/BLL_2019_072
Arslan BA, Isik FB, Gur H, Ozen F, Catal T. Apoptotic Effect of Nigella sativa on Human Lymphoma U937 Cells. Pharmacogn Mag. 2017;13(Suppl 3):628-32. https://doi.org/10.4103/pm.pm_93_17
Mao Z, Gan C, Zhu J, Ma N, Wu L, Wang L, & Wang, X. Anti-atherosclerotic activities of flavonoids from the flowers of Helichrysum arenarium L. MOENCH through the pathway of anti-inflammation. Bioorg Med Chem Lett. 2017;27(12):2812-17.https://doi.org/10.1016/j.bmcl.2017.04.076
Les F, Venditti A, Cásedas G, Frezza C, Guiso M, Sciubba F, Serafini M, Bianco A, Valero, MS, López V. Everlasting flower (Helichrysum stoechas Moench) as a potential source of bioactive molecules with antiproliferative, antioxidant, antidiabetic and neuroprotective properties. Industrial Crops and Products. 2017; 108:295-302. https://doi.org/10.1016/j.indcrop.2017.06.043
Elaguel A, Kallel I, Gargouri B, Amor IB, Hadrich B, Messaoud EB, Gdoura R, Lassoued S, Gargouri A. Lawsonia inermis essential oil: extraction optimization by RSM, antioxidant activity, lipid peroxydation and antiproliferative effects. Lipids Health Dis. 2019;18(1):196. https://doi.org/10.1186/s12944-019-1141-1
Hejazi II, Khanam R, Mehdi SH, Bhat AR, Rizvi MMA, Islam A, Thakur SC, Athar F. New insights into the antioxidant and apoptotic potential of Glycyrrhiza glabra L. during hydrogen peroxide mediated oxidative stress: An in vitro and in silico evaluation. Biomed Pharmacother. 2017;94:265-79. https://doi.org/10.1016/j.biopha.2017.06.108
Badmus JA, Odunola OA, Yekeen TA, Gbadegesin AM, Fatoki JO, Godo MO, Oyebanjo KS, Hiss DC. Evaluation of antioxidant, antimutagenic, and lipid peroxidation inhibitory activities of selected fractions of Holarrhena floribunda (G. Don) leaves. Acta Biochim. Pol. 2013;60:435-42. https://doi.org/10.18388/abp.2013_2004
Czinner E, Lemberkovics É, Bihátsi-Karsai E, Vitányi G, Lelik L. Composition of the Essential Oil from the Inflorescence of Helichrysum arenarium (L.) Moench. J. Essent. Oil Res. 2000;12(6):728-30. https://doi.org/10.1080/10412905.2000.9712202
Judžentienė A, Charkova T, Misiūnas A.Chemical composition of the essential oils from Helichrysum arenarium (L.) plants growing in Lithuanian forests. J. Essent. Oil Res. 2019;31(4):305-11. https://doi.org/10.1080/10412905.2019.1572550
Lemberkovics E, Czinner E, Balázs A, Karsai BE, Vitany G.New data on composition of esssential oil from inflorescence of everlasting (Helichrysum arenarium (L.) Moench.) Acta Pharm. Hung. 2017;1(2):187-91
Rančić A, Soković M, Vukojević J, Simić A, Marin P, Duletić-Laušević S, Djoković D, Chemical composition and antimicrobial activities of essential oils of Myrrhis odorata (L.) Scop, Hypericum perforatum L and Helichrysum arenarium (L.) Moench. J. Essent. Oil Res., 2005;17(3):341-45. https://doi.org/10.1080/10412905.2005.9698925
Judzentiene A, Butkiene R. Chemical composition of the essential oils of wild Helichrysum arenarium (L.) with differently colored inflorescences from Eastern Lithuania. J. Essent. Oil Res. 2006;18(1):80-3. https://doi.org/10.1080/10412905.2006.9699391
Jahantigh F. Edible film based on Vicia faba shell gum, nano silver and Helichrysum arenarium essence, Nutr. Food Sci. 2019; 50:280-7. https://doi.org/10.1108/NFS-04-2019-0136
Judzentiene A, Budiene J, Nedveckyte I, Garjonyte R. Antioxidant and Toxic Activity of Helichrysum arenarium (L.) Moench and Helichrysum italicum (Roth) G. Don Essential Oils and Extracts. Molecules. 2022;27(4):1311. https://doi.org/10.3390/molecules27041311.
Zheljazkov VD, Semerdjieva I, Yankova-Tsvetkova E, Astatkie T, Stanev S, Dincheva I, Kačániová M. Chemical Profile and antimicrobial activity of the essential oils of Helichrysum arenarium (L.) Moench. and Helichrysum italicum (Roth.) G. Don. Plants.2022; 11(7):951. https://doi.org/10.3390/plants11070951.
Kucukoglu O, Ozturk B, Kamataki T, Topcu Z. Inhibitory Activities of Helichrysum Taxa on Mammalian Type I DNA Topoisomerase. Pharmaceutical Biology, 2006;44(3):189-93. https://doi.org/10.1080/13880200600685972
Atalay VE, Asar S. Determination of the inhibition effect of hesperetin and its derivatives on Candida glabrata by molecular docking method. EUCHEMBIOJ. 2024:27–38. https://doi.org/10.62063/ecb-15.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Pharmedicine Journal
This work is licensed under a Creative Commons Attribution 4.0 International License.
