Effects of Geographical Origin and Extraction Methods on the Phenolic Content of Salvia officinalis
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https://doi.org/10.62482/pmj.32Keywords:
Extraction, Salvia officinalis, HPLC, phenolic compoundsAbstract
Introduction: Salvia officinalis L., also known as sage, is a widely used medicinal plant known for its rich phenolic content and associated health benefits. It is known that the phenolic profile of sage varies depending on the geographical origin and extraction method. This study aimed to evaluate the phenolic acid composition of S. officinalis samples collected from two geographical locations, Jordan and Isparta-Türkiye, using three extraction techniques.
Methods: Dried plant materials were extracted using infusion, decoction, and organic solvent extraction (methanol/acetonitrile) and the phenolic content was analyzed analyzed using high-performance liquid chromatography with photodiode array detection (HPLC-PDA). Here, 15 phenolic compounds were screened and evaluated.
Results: In this study, rosmarinic acid appeared as the predominant compound in decoction extracts from both locations (19,442 mg/g in Jordan and 17,485 mg/g in Türkiye, respectively), while epicatechin was the major component in infusion extracts. Organic solvent extracts showed moderate levels of phenolics, with rosmarinic acid being the most abundant.
Conclusions: Both geographical origin and extraction technique affected the phenolic content of S. officinalis. The decoction method was found to be the most effective extraction method for phenolic compounds, indicating superior efficacy in maximizing the health-promoting properties of the plant.
References
Özdemi̇r C, Şenel G. The Morphological, Anatomical and Karyological Propertiesof Salvia sclarea L. Turk J Botany[Internet]. 1999; 23:1(“The Morphological, Anatomical and Karyological Propertiesof Salvia sclarea L.):7-18. 1
Grdiša M, Jug-Dujaković M, Lončarić M, Carović-Stanko K, Ninčević T, Liber Z, Radosavljevic I, Satovic Z. Dalmatian Sage (Salvia offi cinalis L.): A Review of Biochemical Contents, Medical Properties and Genetic Diversity. Vol. 80, Agriculturae Conspectus Scientifi cus. 2015.
Eidi A, Eidi M. Antidiabetic effects of sage (Salvia officinalis L.) leaves in normal and streptozotocin-induced diabetic rats. Diabetes and Metabolic Syndrome: Clinical Research and Reviews. 2009;3(1):40-44. https://doi.org/10.1016/j.dsx.2008.10.007
Mayer B, Baggio CH, Freitas CS, dos Santos AC, Twardowschy A, Horst H, Pizzolatti M, Micke G, Heller M, Santos E, Otuki M, Marquesw M. Gastroprotective constituents of Salvia officinalis L. Fitoterapia. 2009;80(7):421-426. https://doi.org/10.1016/j.fitote.2009.05.015
Ninomiya K, Matsuda H, Shimoda H, Nishida N, Kasajima N, Yoshino T, Morikawa T, Yoshikawa M. Carnosic acid, a new class of lipid absorption inhibitor from sage. Bioorg Med Chem Lett. 2004;19;14(8):1943-1946. https://doi.org/10.1016/j.bmcl.2004.01.091
Tada M, Okuno K, Chiba K, Ohnishi E, Yoshii T. Antiviral diterpenes from Salvia officinalis. Phytochemistry. 1994 Jan;35(2):539-541. https://doi.org/10.1016/S0031-9422(00)94798-8
Todorov S, Philianos S, Petkov V, Harvala C, Zamfirova R, Olimpiou H. Experimental pharmacological study of three species from genus Salvia. Acta Physiol Pharmacol Bulg.1984;10(2):13-20.
Longaray Delamare AP, Moschen-Pistorello IT, Artico L, Atti-Serafini L, Echeverrigaray S. Antibacterial activity of the essential oils of Salvia officinalis L. and Salvia triloba L. cultivated in South Brazil. Food Chem. 2007;100(2):603-608. https://doi.org/10.1016/j.foodchem.2005.09.078
Jedinák A, Mučková M, Košt’álová D, Maliar T, Mašterová I. Antiprotease and Antimetastatic Activity of Ursolic Acid Isolated from Salvia officinalis. Zeitschrift für Naturforschung C. 2006 Dec 1;61(11–12):777-782. https://doi.org/10.1515/znc-2006-11-1203
R. K. M. Hay, P. G. Waterman. Volatile oil crops: their biology, biochemistry and production. Edinburgh,UK: Longman Scientific & Technical; 1993. 97–111 p. https://doi.org/10.1007/BFO2862332
Lu Y, Yeap Foo L. Flavonoid and phenolic glycosides from Salvia officinalis. Phytochemistry. 2000;55(3):263-267. https://doi.org/10.1016/S0031-9422(00)00309-5
Badiee P, Nasirzadeh AR, Motaffaf M. Comparison of Salvia officinalis L. essential oil and antifungal agents against candida species. J Pharm Technol Drug Res. 2012;1(1):7. https://doi.org/10.7243/2050-120x-1-7
Hayouni EA, Chraief I, Abedrabba M, Bouix M, Leveau J-Y, Mohammed H, Hamdi M. Tunisian Salvia officinalis L. and Schinus molle L. essential oils: Their chemical compositions and their preservative effects against Salmonella inoculated in minced beef meat. Int J Food Microbiol. 2008;125(3):242-251. https://doi.org/10.1016/j.ijfoodmicro.2008.04.005
Länger R, Mechtler Ch, Jurenitsch J. Composition of the Essential Oils of Commercial Samples of Salvia officinalis L. and S. fruticosa Miller: A Comparison of Oils Obtained by Extraction and Steam Distillation. Phytochemical Analysis. 1996;7(6):289-293. https://doi.org/10.1002/(SICI)1099-1565(199611)7:6<289: AID-PCA318>3.0.CO;2-7
Lima CF, Valentao PCR, Andrade PB, Seabra RM, Fernandes- Ferreira M, Pereira-Wilson C. Water and methanolic extracts of Salvia officinalis protect HepG2 cells from t-BHP induced oxidative damage. Chem Biol Interact. 2007;167(2):107-115. https://doi.org/10.1016/j.cbi.2007.01.020
Ghorbani A, Esmaeilizadeh M. Pharmacological properties of Salvia officinalis and its components. J Tradit Complement Med. 2017;7(4):433-440. https://doi.org/10.1016/j.jtcme.2016.12.014
Francik S, Francik R, Sadowska U, Bystrowska B, Zawiślak A, Knapczyk A, et al. Identification of Phenolic Compounds and Determination of Antioxidant Activity in Extracts and Infusions of Salvia Leaves. Materials. 2020;19;13(24):5811. https://doi.org/10.3390/ma13245811
Kamatou GPP, Makunga NP, Ramogola WPN, Viljoen AM. South African Salvia species: A review of biological activities and phytochemistry. J Ethnopharmacol. 2008;119(3):664-672.https://doi.org/10.1016/j.jep.2008.06.030
Li Y, Zidorn C. Seasonal variations of natural products in European herbs. Phytochemistry Reviews. 2022;10;21(5):1549-1575. https://doi.org/10.1007/s11101.021.09797-7
Garcia CSC, Menti C, Lambert APF, Barcellos T, Moura S, Calloni C, BrancO C, Salvador M, Ely-Roesch M, Henriques JA. Pharmacological perspectives from Brazilian Salvia officinalis (Lamiaceae): antioxidant, and antitumor in mammalian cells. An Acad Bras Cienc. 2016; 2;88(1):281-292. https://doi.org/10.1590/0001.376.5201520150344
Tohma H, Köksal E, Kılıç Ö, Alan Y, Yılmaz M, Gülçin İ, Bursal E, Alwasel S. RP-HPLC/MS/MS Analysis of the Phenolic Compounds, Antioxidant and Antimicrobial Activities of Salvia L. Species. Antioxidants. 2016;21;5(4):38. https://doi.org/10.3390/antiox5040038
Stalikas CD. Extraction, separation, and detection methods for phenolic acids and flavonoids. J Sep Sci. 2007;11:30(18):3268-3295. https://doi.org/10.1002/jssc.200700261
Ghasemzadeh A, Jaafar HZE, Rahmat A. Synthesis of Phenolics and Flavonoids in Ginger (Zingiber officinale Roscoe) and Their Effects on Photosynthesis Rate. Int J Mol Sci. 2010; 15:11(11):4539-4555. https://doi.org/10.3390/ijms11114539
Sharma Y, Velamuri R, Fagan J, Schaefer J. UHPLC-ESI- QTOF-Mass Spectrometric Assessment of the Polyphenolic Content of Salvia officinalis to Evaluate the Efficiency of Traditional Herbal Extraction Procedures. Revista Brasileira de Farmacognosia. 2020;30(5):701-708. https://doi.org/10.1007/s43450.020.00106-5
Jakovljević M, Jokić S, Molnar M, Jerković I. Application of Deep Eutectic Solvents for the Extraction of Carnosic Acid and Carnosol from Sage (Salvia officinalis L.) with Response Surface Methodology Optimization. Plants. 202; 2:10(1):80. https://doi.org/10.3390/plants10010080
Durling N, Catchpole O, Grey J, Webby R, Mitchell K, Foo L, Perry, B. Extraction of phenolics and essential oil from dried sage (Salvia officinalis) using ethanol–water mixtures. Food Chem. 2007;101(4):1417-1424. https://doi.org/10.1016/j.foodchem.2006.03.050
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